Semi-automated method for the differential determination of plasma catecholamines.

نویسنده

  • H McCullough
چکیده

A method is described for the estimation of adrenaline and noradrenaline in a 5 to 10 ml plasma sample.A batch technique of adsorption and elution from alumina is used and the final estimation is carried out using an automated fluorimetric technique based on the trihydroxyindole method. Fluorescence is stabilized first by thioglycollic acid to give noradrenaline concentrations only and then by ascorbic acid to give the total catecholamine concentration. The results of a number of catecholamine analyses are given.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Automatic semi-microcolumn liquid chromatographic determination of catecholamines in rat plasma utilizing peroxyoxalate chemiluminescence reaction.

A fully automated and highly sensitive method with a semi-microcolumn liquid chromatography system for the determination of rat plasma catecholamines (CAs) was developed. Automated on-line extraction of CAs in diluted plasma using a precolumn packed with strong acidic cation exchange resin was coupled with separation of CAs on a semi-microcolumn (250 x 1.5 mm id). fluorogenic derivatization wit...

متن کامل

Development of a Simple and Sensitive Terbium Sensitized Fluorescence Method for Determination of Epinephrine and Norepinephrine in Serum

     A simple, rapid, selective and highly sensitive fluorimetric method for determination of two catecholamines, i.e. norepinephrine (NE) and epinephrine (EP), in serum samples was developed. The method is based on the fluorescence sensitization of terbium (Tb3+) by complexation with both catecholamines in the presence of lanthanum (La3+), as a co-cation, and in a Na...

متن کامل

Semi-automated method for the determination of free fatty acids in plasma.

A semi-automated method for the determination of free fatty acids in plasma based on Mosinger's manual method (Mosinger, 1965) is described and the results of a comparison with Dole's titrimetric method (Dole, 1956) are presented. There is almost perfect correlation between the two methods, and the automated technique is simple, reliable, and significantly more accurate and precise than the tit...

متن کامل

Automated analysis for plasma epinephrine and norepinephrine by liquid chromatography, including a sample cleanup procedure.

We describe a fully automated method of analysis for plasma epinephrine and norepinephrine by use of a two-column system of "high-performance" liquid chromatography. Catecholamines in deproteinized plasma are purified on the first (preparation) column, then transferred automatically to the second (analytical) column in which epinephrine and norepinephrine are resolved. These compounds are then ...

متن کامل

Computational Aided-Molecular Imprinted Polymer Design for Solid Phase Extraction of Metaproterenol from Plasma and Determination by Voltammetry Using Modified Carbon Nanotube Electrode

A molecular imprinted polymer (MIP) was computationally designed and synthesized for the selective extraction of metaproterenol (MTP), from human plasma. In this regards semi empirical MP3 and mechanical quantum (DFT) calculations were used to find a suitable functional monomers. On the basis of computational and experimental results, acrylic acid (AA) and DMSO:MeOH (90:10 %V/V) were found to b...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical pathology

دوره 21 6  شماره 

صفحات  -

تاریخ انتشار 1968